Isolation and diagnostic of Clostridium histolyticum from wounds with sharp objects, explosions and Splinters

A total of 75 samples (25 wound swabs with sharp objects , 25 wound swabs from explosions and 25 wound swabs from Splinters ) of patients at many age group range from 15 to 40 year old were collected from patients who attending the Samawah Teaching Hospital for pediatrics and Gynecology of AL-Muthanna governorates; Through the period which extended from February 2014 to January 2015. The isolation and identification methods of Clostridium histolyticum were followed upon the morphological, cultural and biochemical characteristics. The phenotypic results showed that the isolation percent percent of clostridium histolyticum were (56% , 84%, 68% ) from wound swabs with sharp objects, explosions and Splinters respectively.