17Apr 2022

ENHANCED EFFECTS OF CHOLECALCIFEROL PLUS PIOGLITAZONE ON LIPOLYSIS BY UP-REGULATION OF PPAR-ALPHA MRNA IN MATURE 3T3-L1 ADIPOCYTES

  • Centro de Investigaciones Biomedicas, Universidad Veracruzana, Av. Luis Castelazo Ayala S/N, Col. Industrial Animas, Xalapa, Veracruz, Mexico. CP. 91190.
  • Biomedicine in public health Laboratory, Public Health Institute, Universidad Veracruzana. Av. Luis Castelazo Ayala S/N, Col. Industrial Animas, Xalapa, Veracruz CP. 91190, Mexico.
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Introduction:Vitamin D deficiency has been associated with obesity, diabetes mellitus type 2, and hypertension diseases. The active vitamin D binds to nuclear vitamin D receptor (VDR) and regulating lipids and carbohydrates metabolism genes however, prophylaxis is ruled out because induce hypercalcemia and hyperphosphatemia. VDR and the peroxisome proliferator-activated receptor gamma (PPARγ) cross-talk regulates lipid and glucose metabolism genes.

Methods: In this study was analyze the effect of cholecalciferol (CCF) as vitamin D plus pioglitazone (PIO) on mature 3T3-L1 adipocytes on proliferation, fat accumulation by colorimetric methods and the mRNA genes involved in lipid signaling by RT-PCR.

Results: cellular proliferation in mature 3T3-L1 adipocytes treated with CCF plus PIO combination during 0.5, 6 and 24 hours, were not affected however, high CCF concentrations decreased cell proliferation. The combined treatment decreased fat accumulation, as well as increases glycerol release with CCF plus PIO-treated adipocytes. Key adipogenic genes were down-regulated (CEBP-α, SREBP-, PPARᵞ) whereas lipogenic genes (PPAR-α) were up-regulated at mRNA level.

Conclusion: These findings imply that CCF plus PIO induce lipolysis through the expression of PPAR-alpha in adipocytes.

[Hernandez-Jimenez Janeth, Arroyo-Xochihua Omar, Mendoza-Soto Carlos, Coutino-Rodriguez Rocio and Arroyo-Helguera Omar (2022); ENHANCED EFFECTS OF CHOLECALCIFEROL PLUS PIOGLITAZONE ON LIPOLYSIS BY UP-REGULATION OF PPAR-ALPHA MRNA IN MATURE 3T3-L1 ADIPOCYTES Int. J. of Adv. Res. 10 (Apr). 641-650] (ISSN 2320-5407). www.journalijar.com

Omar Arroyo-Helguera
Instituto de Salud Pública, Universidad Veracruzana
Mexico

DOI:

Article DOI: 10.21474/IJAR01/14596      
DOI URL: https://dx.doi.org/10.21474/IJAR01/14596

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