Identification of blaOXA-1 genes in Klebsiella isolated from urinary tract infections.

The study aimed to evaluate the distribution of blaoxa-1 genes which coding for oxacillinsaes ?- lactamases which hydrolyzed oxacillin and cloxacillin ?- lactams . All urine samples taken from patients complained from urinary tract infections which collected during year 2014 in Hospitals of Al-Najaf province were cultured on MacConkey agar were the results revealed that 250 of isolates were lactose fermentative versus to 50 of isolates were lactose non fermentative. Conventional tests IMViC (Indol, Methyl red, Voges- Proskauer and Citrate utilization) were implemented for preliminary identification of isolates, further more motility test was used crucially. Lactose fermentative and non motile isolates were candidated to be long-established by Vitek 2 system. The results revealed that 53 of isolates identified as Klebsiella spp. The results of molecular study showed the prevalence of blaoxa genes in 38(71.7%) of isolates which been screened by Polymerase Chain Reaction .