Vol. 3 (03)

PROTOCOL FOR QUICK ISOLATION AND PURIFICATION OF CAPSICUM PROTOPLASTS USING LOWER CONCENTRATIONS OF MACEROZYME AND CELLULASE

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Abstract

Capsicum protoplast isolation protocols till to date is involved prolonged incubation with higher concentration of macerozyme and cellulase in the digestion solution. In this study we have developed a protocol for quick (4½ h) isolation of protoplasts using lower concentration of macerozyme and cellulase, further we developed a simple protocol for purification of protoplasts. We obtained 5.8 x 105 Protoplasts/ gram yield by using lower concentrations of macerozyme (0.2%) and cellulase (1%).

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SYAM PRASAD SURA, VIJAYAPAL REDDY BAIREDDY, MURALIKRISHNA NARRA, SADANANDAM ABBAGANI (2015); PROTOCOL FOR QUICK ISOLATION AND PURIFICATION OF CAPSICUM PROTOPLASTS USING LOWER CONCENTRATIONS OF MACEROZYME AND CELLULASE, Int. J. of Adv. Res., 3 (03), 0, ISSN 2320-5407.

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SYAM PRASAD SURA

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