Validated Derivative Spectrophotometric Estimation of Pantoprazole in Bulk and Tablet Dosage form.

A simple and sensitive First order derivative spectrophotometric method has been developed for the estimation of Pantoprazole both in pure form and in pharmaceutical solid dosage form. Absorption maxima of Pantoprazole in 0.1N sodium hydroxide was found to be at 286.2 nm. Beer’s law is obeyed in the range 4-20 ?g/mL. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The percentages assay of Pantoprazole in tablet was more than 99%. The method was validated by determining its sensitivity, accuracy and precision which proves suitability of the developed method for the routine estimation of Pantoprazole in bulk and Pharmaceutical solid dosage form.