Activity of two serine proteases and anti-bacterial studies on both excretory/secretory products and whole body homogenates of third instar larvae of Chrysomya megacephala

The present study revealed the activity of both trypsin and chymotrypsin in both excretory/secretory products and homogenates of whole body larvae of third instars larvae of Chrysomya megacephala. The optimum pH activity for trypsin and chymotrypsin enzymes was at pH 9 in the excretory/secretory products and for both of them and at pH 7 in homogenates of whole body larvae for the two enzymes. The present study also evaluate the potential anti-bacterial activity of both excretory/secretory products and homogenates of whole body larvae of third instars’ larvae of Chrysomya megacephala against gram -ve bacteria (Escherichia coli,Pseudomonas aeruginosa) and gram +ve bacteria (Staphylococus aureus,Bacillis subtilis) The excretory/secretory products showed antibacterial activity against Escherichia coli, Staphylococus aureus,Bacillis subtilis. The minimum inhibitory concentrations (MIC) were 125ug/ml, 31.25ug/ml and 31.25ug/ml respectively. The homogenates of whole body larvae showed antibacterial activity against Escherichia coli, Staphylococus aureus,Bacillis subtilis.The minimum inhibitory concentrations (MIC) was 125ug/ml,31.25ug/ml,15.63ug/ml, respectively. Both the excretory/secretory products and homogenates of third instars larvae of Chrysomya megacephala have no effect on Pseudomonas aeruginosa.