SEMI-QUANTITATIVE ANALYSISOF NUCLEIC ACIDS ON AGAROSE GELS WITH CHOICE OF BEVERAGES AS ELECTROPHORETIC BUFFER
Hyacinthe Tuyubahe
, Venkatanagasai Raju Alluri
, Aarya Harshal Suryawanshi
, Shanmukh Sri Surya Allu
, Murengezi William
, Huma Parveen
and Reethika Singh Ranwas
- Department of Biotechnology, KoneruLakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, India.
- Department of Microbiology, GITAM University, Visakhapatnam, Andhra Pradesh, India.
- Department of Biotechnology, Anwar Ul Uloom College, Hyderabad, Telangana, India.
- Department of Molecular Biology, CresentBiosciences, ICRAB Campus, Kanpur, Uttar Pradesh, India.
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The pH of beverages is known to be low, and has therefore been involved in the increasing acidity incidence. In this study,to report on a rapid and cost-effective approach for nucleic acid characterization using different running buffers for agarose gel electrophoresis.Commercial DNA is used in electrophoretic analysis using a variety of beverages by optimizing its pH value.The results suggest that Royal Stag has little effect on nucleic acid research, while Peter Scot shows high concentration and sensitivity in nucleic acid characterization.Royal Stag drink has revealed that concentration, time, and voltage have little effect on nucleic acid research, although Peter Scot has reported high concentration and sensitivity in nucleic acid characterization. We conclude that this approach is simple, easy, and cost-effective compared to using TAE or TBE as running buffers in electrophoresis of the agarose gel.
[Hyacinthe Tuyubahe, Venkatanagasai Raju Alluri, Aarya Harshal Suryawanshi, Shanmukh Sri Surya Allu, Murengezi William, Huma Parveen and Reethika Singh Ranwas (2023); SEMI-QUANTITATIVE ANALYSISOF NUCLEIC ACIDS ON AGAROSE GELS WITH CHOICE OF BEVERAGES AS ELECTROPHORETIC BUFFER Int. J. of Adv. Res. 11 (Apr). 281-284] (ISSN 2320-5407). www.journalijar.com
Cresent Biosciences
India
