Invitro Callus induction and Root regeneration through the mediation of Agrobacterium rhizogenes in Punica granatum
- Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Agricultural University, Rahuri 413- 722 Dist. Ahmednagar, M.S., India
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Abstract
In Punica granatum highly efficient and reproducible callus and root induction has been developed through A. rhizogenes by using juvenile segment as explant source. One month old calli were treated with A. rhizogenes for root induction in MS medium without auxins. The best roots were derived from callus of pomegranate through the mediation of A.rhizogenes. This protocol opens up prospects for using biotechnological approaches for pomegranate improvement.
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Nisha M. Patil and S.G.Borkar (2015); Invitro Callus induction and Root regeneration through the mediation of Agrobacterium rhizogenes in Punica granatum, Int. J. of Adv. Res., 3 (05), 162-165, ISSN 2320-5407.
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