CHLORPYRIFOS INDUCED HISTOLOGICAL AND BIOCHEMICAL CHANGES IN THE LIVER AND KIDNEY TISSUES OF FEMALE WISTAR RATS

Chlorpyrifos (CPF) is a widely used organophosphorous insecticide in agricultural and domestic platforms. In our previous studies we observed a remarkable toxicity of CPF in the reproductive organs of female Wistar rats. To study effect of CPF on liver and kidney two groups of Wistar albino rats were dosed orally for 8 weeks with chlorpyrifos in vegetable oil (0.1mg/kg bw and 2.5mg/kg bw) and control group was given only vegetable oil. A significant (P<0.05) increase in total plasma proteins in rats treated with higher dose (2.5 mg/kg bw) was observed. A non-significant increase in liver total proteins was observed in treated rats as compared to control but there was a significant (P<0.01) decrease in the liver glycogen valuein higher dose (2.5 mg/kg bw) group. No significant change in blood urea value was found in the treated rats as compared to control. A significant (P<0.01) increase in liver alanine aminotransferase and lactate dehydrogenase enzyme activity was observed in both the treated groups as compared to control.Degenerative histological changes like leucocyte infiltration, proliferation in Kupffer cells, dilated blood sinusoids and cytoplasmic vacuolization in liver and kidney were observed in the treated rats. The results show that the sub-chronic exposure of CPF affects liver and kidney adversely.