30May 2015

Lactic Acid Bacteria: Antimicrobial activity and in vitro, in vivo studies of LAB activity on Fusarium oxysporum infected tomato seeds

  • 1. Assistant Professor, Department of Biotechnology, P.V.P. College of Arts, Science & Commerce, Pravaranagar, Loni – 413713, Ahmednagar (Dt.), Maharashtra.
  • 2. Department of Biotechnology, P.V.P. College of Arts, Science & Commerce, Pravaranagar, Loni – 413713, Ahmednagar (Dt.), Maharashtra
  • 3. Department of Biotechnology, P.V.P. College of Arts, Science & Commerce, Pravaranagar, Loni – 413713, Ahmednagar (Dt.), Maharashtra
  • Assistant Professor, Department of Biotechnology, P.V.P. College of Arts, Science & Commerce, Pravaranagar, Loni – 413713, Ahmednagar (Dt.), Maharashtra
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Lactic Acid Bacteria (LAB) being a group of microorganisms, are gram positive, acid-tolerant, non-sporulating cocci or bacilli. The study of lipolytic and proteolytic activities of LAB is essential to understand the biological properties of LAB. Currently, the antimicrobial activity of LAB is of major concern in plant biology. It is necessary to study the antimicrobial activity of LAB to establish a standard methodology for LAB utilization in Drug development. The current study aims to isolate and characterize LAB, to check their lipolytic, proteolytic, antimicrobial activities along with their antibiotic sensitivity. The antifungal activity of LAB on Fusarium oxysporum has been observed in vitro and in vivo. The results showed that, LAB has no lipolytic activity on Tween-20 and showed proteolytic activity. LAB showed antimicrobial activity for P. aeruginosa, S. typhi, E. coli, B. subtilis and C. albicans. LAB showed antibiotic sensitivity for Ampicillin, Amoxicillin, Nitrofurantion, Ciprofloaxin, Cotrimoxazole, Streptomycin, tetracycline, Pencillin, Kanamycin and resistance for gentimycin and colistin. The in vitro studies showed that LAB has 72.42% inhibitory property over F. oxysporum growth, indicating that they control the fungal action. The in vivo studies showed that Fusarium has the capacity to suppress LAB activity during seed germination

[Kavita Sarjerao Dhamale, Pragati Devidas Sonawane, Ashvini Suryakant Jaybhaye, Pavan Chand Akkiraju (2015); Lactic Acid Bacteria: Antimicrobial activity and in vitro, in vivo studies of LAB activity on Fusarium oxysporum infected tomato seeds Int. J. of Adv. Res. 3 (May). 954-963] (ISSN 2320-5407). www.journalijar.com

Dr. Pavan Chand Akkiraju

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