Dynamic of Proteomic Responses on Wheat Seed before Germination

As protein metabolism plays an important role in plant adaptation to growth, this study was designed to identify protein expression in wheat seed at 0h,3h,17h,25h before germination. Proteins were extracted and separated by running two-dimensional gel electrophoresis (2-DE), which allowed the identification of some significantly different gel spots. The spots were further verified by matrix-assisted laser desorption/ionization-time of flightmass spectrometry, in which they were confirmed to be wheat seeds proteins. The results showed: (i) On 0h,3h,17h,25h, the number of protein spots which could be identified were 231,233,232,231 respectively. (ii) 4 protein spots were identified using mass spectrometry They were further verified as nucleoside diphosphate kinase?(NDKP?), 17.5ku heat shock protein (HSP), ATP synthase and LEA protein 27. These data support the assumption that outer environment may have a regulatory role on protein metabolism of wheat seed and make it to regulatory protein expression to adapt the changed environment.