Expressions of IFN-? and IL-2 in Biliary Atresia Mice Model After Exposure with Rhesus Rotavirus (RRV)

Background: Biliary atresia (BA) is a cholangiopathic obstructive disorder with unclear etiology and pathogenesis . It was thought that BA is originated from bile duct epithelium viral infection, followed by immunological processes and ended with biliary cirrhosis. Objective: To determine the effect of induction and duration of illness after rhesus rotavirus (RRV) exposure to changes IFN-? and IL-2 in mice model of BA. Methods: Fourty-eight babies from 20 pregnant Balb/c mice were randomized into 2 groups that received placebo or 1.5 x106 PFU RRV intraperitoneally within less than a day after birth. Each group was terminated on day 3, 7, 14 and 21, further examined the expression of IFN-? and IL-2 with flowcytometer. Statistical analysis using Mann Whitney and Kruskal Wallis . Result: Differences in the expression of IFN-? and IL-2 day 3, 7, 14 and 21. IFN-? day 3 control 1.72 (0.05) vs trial groups 2.66 (0.05) p=0.020, day 7 0.86 (0.07) vs 1.64 (0.05) p=0.011, day 14 2.66 (0.08) vs 97.22 (1.27) p=0.006, day 21 10.14 (0.84) vs 29.45 (1.05) p=0.011. Each of them differ significantly with control p<0.001 and trial groups p=0.002. Expression of IL-2 day 3 1.15 (0.04) vs 2.73 (0.09) p=0.021, day 7 1.66 (0.17) vs 2.93 (0.09) p=0.011, day 14 2.97 (0.20) vs 5.31 (1.19) p=0.006, day 21 5.41 (0.86) vs 29.43 (1.04) p=0.011. Each of these differ significantly with control p<0.001 and trial groups p=0.002. Conclusions: Induction and duration of illness after RRV exposure influence the expression of IFN-? and IL-2 in the mice model of biliary atresia.