EFFECT OF DIFFERENT HORMONE COMBINATIONS ON CALLUS INDUCTION AND PLANT REGENERATION OF STRAWBERRY
- Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh.
- Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh.
- Department of Agricultural Chemistry, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh.
- Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh.
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An experiment was undertaken to examine the effects of different combinations of plant growth regulators for callus induction and plantlet regeneration in Strawberry (Fragaria x ananassa Duch.). Leaf discs derived from two months old strawberry plants were cultured on MS media supplemented with BAP. The cultures were incubated for 4 weeks in dark followed by another 4 weeks under 16/8 hr light regime. The effects of different concentrations of BAP (0, 1.5, 3.0 and 6.0 mg L-1) on callus induction were investigated. Among the concentrations 3.0 mg L 1 BAP showed the highest percentage (93.33%) of callus induction. To regenerate shoots, the calli derived from leaf discs were cultured on shoot induction media containing different combinations and concentrations of BAP (0, 1.5, 3.0 and 6.0 mg L-1 ) and GA3 (0.5, 1.0, 1.5 and 2.0 mg L-1). The highest percentage of shoot regeneration (93.33%) and number of shoots (15.00) per leaf disc was found to be induced on the MS medium supplemented with 3.0 mg L 1 BAP and 0.5 mg L 1 GA3. The highest (83%) percentage of rooting in shoots was observed in MS medium in combination with 1.5 mg L 1 GA3 and 1.0 mg L 1 IBA.
[WALIUR RAHMAN, SADIATUZ ZOHORA, AMINUL ISLAM TALUKDER, OMAR KAYESS (2015); EFFECT OF DIFFERENT HORMONE COMBINATIONS ON CALLUS INDUCTION AND PLANT REGENERATION OF STRAWBERRY Int. J. of Adv. Res. 3 (Jun). 1244-1250] (ISSN 2320-5407). www.journalijar.com
