Environmental, efface and filed study for source Brucella disease

Objective: Diagnosis of brucellosis from patients suspects of infection and their casualty for brucellosis by serological methods (Rose Bengal test) and culture method e in diagnosis of human brucellosis. Duration and place of study: Blood and serum samples were obtained from suspected and casualty brucellosis patients, referred to many hospitals in different city Baghdad province (Karkh and Rusafa), which include: {General hospital Mohammad Baqir Al-Hakim, Al-Shaheed Al-Sadder hospital, Al-Imam Ali (peace be upon him) hospital}, as well as access to statistics and maps in all Iraq provinces of from Ministry of Health / Communicable Disease Control Center, during the duration from (March to December 2014). Methodology: A total of 117 peripheral blood samples were from patient's suspect of infection and their casualty for brucellosis. The diagnosis of brucellosis was established by clinical findings confirmed by serological test (Rose Bengal test) and culture and confirmed by used Gram staining and different biochemical test for diagnosis of brucellosis. Results: A total of 117 peripheral blood samples, 70 (59.82%) samples were positive result by RBT and 59 (50.42%) samples were positive result by culture was applied to patient's blood. Conclusions: These results indicate that patients were contact with infected livestock or suspected infection of Brucella like: sheep, goat, cow and buffalo located in epidemiological regions in Iraq especially in Baghdad province across the study period and showed blood culture method is important for the detection of brucellosis compared with serological methods (Rose Bengal test) for the diagnosis of brucellosis.