Molecular characterization of selected diazotrophic cyanobacteria of North East India for phylogenetic study

  • National Repository for Cyanobacteria and Microgreen algae (Freshwater), Microbial Resources Division, Institute of Bioresources and Sustainable Development (A National Institute of Department of Biotechnology, Govt. of India), Takyelpat, Imphal-795001, Manipur, India.
  • Department of Life Science and Bionformatics, Assam University, Silchar-788011, Assam, India.
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In the present investigation, 05 strains viz. Anabaena sp. BTA650, Calothrix sp. BTA265, Scytonema hofmanni BTA124, Rivularia sp. BTA510 and Nostoc sp. BTA197 exhibiting high nitrogenase activity were procured from National Repository for Cyanobacteria and Microgreen algae (Freshwater) which were initially isolated from the unexplored niches of North East India. On pairwise similarity alignment by BLASTN, Scytonema hofmanni BTA124 showed 93% identity with Scytonema hofmanni PCC 7110, Nostoc sp. BTA197 showed 99% with Nostoc sp. HA4356-MV1. Calothrix sp. BTA265 showed 99% with Calothrix sp. PCC 7715. Rivularia sp. BTA510 showed 89% with Rivularia sp. MU24 UAM-305. Anabaena sp. BTA650 showed 97% with Anabaena sp. 08-05 with E value (0.0) for all the strains. The GenBank accession numbers for the 16S rDNA sequences determined in the study are KF779147, KF779148, KF779149, KF779151 and KF779152 for Scytonema hofmanni BTA124, Nostoc sp. BTA197, Calothrix sp. BTA265, Rivularia sp. BTA510 and Anabaena sp. BTA650 respectively.

[Gunapati Oinam, A. Thadoi, K. Ojit Singh, O. Avijeet Singh, Th. Indrama, Laxmipriya Koijam, Wangkhem Indira, Chungkham Silvia, A. Subhalaxmi Sharma, Romi Khangembam, Minerva Shamjetshabam, Th. Bidyababy, K. Sarabati, O.N. Tiwari and G.D. Sharma (2015); Molecular characterization of selected diazotrophic cyanobacteria of North East India for phylogenetic study Int. J. of Adv. Res. 3 (Aug). 261-271] (ISSN 2320-5407). www.journalijar.com

Gunapati Oinam

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