Comparative Study of Molecular Markers of P.aeruginosa Isolated From Clinical and Environmental

The present study includes (380) samples collected from clinical and environmental sources (clinical ) ones included patients suffering from various infection and environmental samples . All samples were inoculated on selective media to detect Pseudomonas aeruginosa isolates . Out of the 380 samples, only 116(30.5%) isolates belonged to P. aeruginosa, identified by diagnostic tests including cultural characters, biochemical tests and confirmed by PCR technique . The distribution of these isolates were 61(62.0%) burn, 9 (9.2%), urine, 3(3.1), blood, 5(5.1%), sputum , 18(18.3%( ear swab , and 1(1.0%) each pleural and C.S.F, while environmental samples were 5(27.8) water , 3(16.7) soil, and 10(55.5%) sewage .Regarding to molecular diagnosis , the outcome showed molecular detection revealed that 116 isolates of P. aeruginosa were 16SrRNA gene ( which a specific of P. aeruginosa ) . Genotypic detection of pathogenicity markers genes of P.aeruginosa were carrying (plc-H, plc-N) as 89 (96%) for clinical isolate and (13(72%),14(78%) for environmental isolate, so the proportion of possession of rhIAB was as the following 89(96%) ,