Detection of CTX-M and CTX-M1 Genes in Klebsiella spp. Isolation from Clinical and Environmental Samples in Thi-Qar Province.
- Nasyhria Technology Institute.
- Collage of Science /Thi-Qar University
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The aim of this study was to identify the prevalence of some extended-spectrum ?-lactamases genes (ESBLs) (blaCTX-M , blaCTX-M1) in Klebsiella spp. isolated from clinical specimens ( Burns , wounds , urinary tract infections, respiratory tract infections and otitis media patients and vaginal infection ) and hospitals environments . A total of 554 samples (468 clinical and 86 environmental samples) during the period from October 2013 to March 2014 were collected. Only 45(8.1%) samples were gave positive growth for as following: 2 ? 48 (4 %) from burns , 7? 40 (17 %) from wounds , 29 ?315 ( 9.20%) from urinary tract infections , 4 ? 25 (16%) from respiratory tract infections , 3 ?86 (3,48%) from hospitals environments. All the 45 isolates were submitted to molecular detection of some MBLs genes (blaCTX-M , blaCTX-M1) by using PCR technique . The results showed that only 28 (62,22%) isolates were carried these genes. and 21 isolates were carried both genes.
[Yahya A. Abbas, Zainab Mohammad Edi (2015); Detection of CTX-M and CTX-M1 Genes in Klebsiella spp. Isolation from Clinical and Environmental Samples in Thi-Qar Province. Int. J. of Adv. Res. 3 (Aug). 1012-1019] (ISSN 2320-5407). www.journalijar.com
