Silver nitrate is essential for successful regeneration of Egyptian sunflower (Helianthus annus L.) cv. Giza 102
- Oil Crops Biotechnology Lab, Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, 12619. Egypt.
- Department of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt.
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Sunflower (Helianthus annus L.) is an important source of vegetable oil. Egypt relies almost entirely on importation of vegetable oils due to limitation of land and competition with other warm-season more profitable crops. In the present study, cotyledons dissected from 24 hr germinating seeds were tested on 16 different media. The highest shoot-buds formation was on medium fortified with 0.5 mg/l naphthalene acetic acid and 1.0 mg/l benzyl adenine, yet no further development was observed. Healthy shoots only developed after adding 5.0 mg/l silver nitrate to the regeneration medium; while shoot elongation was achieved on a medium fortified with 0.05 mg/l indol acetic acid and 5.0 mg/l silver nitrate. Root formation was possible when using a low-salt medium with indol acetic acid and charcoal. The current work is a step in the production of genetically enhanced dehydration-stress tolerance varieties suitable to new reclaimed area in Egypt.
[Shaimaa R.M. Khalil, Bassita A. Hussein, Ebtissam H. A. Hussein, Mohamed S. Tawfik (2015); Silver nitrate is essential for successful regeneration of Egyptian sunflower (Helianthus annus L.) cv. Giza 102 Int. J. of Adv. Res. 3 (Sep). 506-512] (ISSN 2320-5407). www.journalijar.com
