PHYTOCHEMICAL CHARACTERIZATION AND IN VITRO EVALUATION OF ANTISICKLING, ANTICOAGULANT, AND ANTI INFLAMMATORY ACTIVITIES OF BARK EXTRACTS FROM ENANTIA CHLORANTHA AND ALSTONIA BOONEI

Background: Sickle cell disease (SCD) is a major hereditary hemoglobinopathy in sub Saharan Africa. In Gabon, Enantia chlorantha and Alstonia boonei are widely used in ethnomedicine for managing sickle cell crises. Objective: To conduct phytochemical analysis and evaluate antioxidant, anti inflammatory, antisickling, and anticoagulant activities of aqueous bark extracts of E. chlorantha and A. boonei in the context of traditional treatment of SCD. Methods: Phytochemical screening and quantification of phenolic compounds were performed using standardized protocols. Antioxidant activity was assessed by DPPH radical scavenging and phosphomolybdenum assays. Anti inflammatory potential was evaluated through protein denaturation and red blood cell hemolysis inhibition assays. Antisickling and anticoagulant activities were determined using the Emmel and Lee & White methods.