Recent advances in the identification of the microbiota involved in Endo-Perio Lesions -A literature review

The aim of this paper is to discuss the communications between the pulp and periodontium, microorganisms involved in endo-perio lesions and the variousmolecular biology techniques available for more comprehensive broad-range investigation of the bacterial communities in endo-perio infections.This review also examines the recent approaches of themolecular principles, providing us with further information on the hidden metabolic diversity of endo-periomicrobiota. Various revolutionary techniques such as Polymerase Chain Reaction (PCR),Immunofluorescent assays, hybridization assays such as Checkerboard. Fluorescence in situ hybridization (FISH), DNA probes and metagenomic studies provides a promising strategy to study the full genetic pool and will provide an evidence based research revealing factors that can contribute to efficient treatment of endo-perio lesions.