CYCLIC UREA ANALOGUES AS INHIBITORS OF JACK BEAN UREASE.
- Department of Chemistry, University of Delhi, New Delhi-110007, India.
- Custom House Laboratory, Custom House, Vasco-Da-Gama, Goa, India
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Based on the structural homology with urea, the substrate of urease, a variety of cyclic urea analogues viz. barbiturate, imidazolidinone and imidazolone derivatives were tested for their effect on jack bean urease in 50mM phosphate buffer, pH=7.0. These compounds were found to inhibit jack bean urease in a concentration dependent manner IC50 varies from 0.6 mM to 3.5mM with no further time dependent changes in the reaction velocities. Evaluation of the kinetic data from Lineweaver-Burk plots and Dixon plots revealed that the analyzed compounds act as reversibile competitive inhibitors of the plant enzyme.
[Jitender M. Khurana, Vandana Sharma, Devanshi Magoo and Subhash Chandra. (2016); CYCLIC UREA ANALOGUES AS INHIBITORS OF JACK BEAN UREASE. Int. J. of Adv. Res. 4 (Jul). 1226-1235] (ISSN 2320-5407). www.journalijar.com
