Dyes decolorization by spore-bound laccase from local isolate of Bacillus subtilis

B. subtilis growth on production medium resulted in spores-bound laccase activity (439.23) U/ml.The optimum pH of spore-bound laccase activity was 6.8 and retained its initial activity at pH 6.8 after incubation for 1 hour at 37?C. The optimum temperature was at 40 ?C and retained its initial activity after 3 hours when incubated at temperature ranged (10-50)°C.The spore-bound laccase retained 100% of its activity after 4 days.Dyes decolorization on solid medium tested by a halo formed around the growth area for all dyes used except for Methyl orange.Dyes decolorization by spore-bound laccase showed that the dyes (eriochrome black T, crystal violet, azure B) were completely decolorized in all concentrations within 10 minutes while Textile (blue), Methyl violet and Methyl orange dyes were decolorized in different percentage.