HISTOPATHOLOGICAL CHANGES CAUSED BY PONGAMIA PINNATA AQUEOUS LEAF EXTRACT IN THE GILL AND INTESTINE OF COMMON CARP- CYPRINUS CARPIO

Studying the toxicity of biopesticidal plant Pongamia pinnata aqueous leaf extract to common carp – Cyprinus carpio. 96 hr Lc50 value was 1300 ppm. The fishes were exposed to sub lethal concentration i.e., 1/4th of Lc 50 value i.e., 350 ppm for a period of one week. After the completion of 24 hrs and 7 days the gill and intestine were dissected out, processed and sectioned at 4µm and stained with H & E stain. The slides were then observed under 40x magnification. The exposed gill showed histopathological changes such as distorted, shortened, twisted and curled secondary gill lamellae with some Vacuolation. The exposed intestine showed pathological changes such as Vacuolation of villi epithelium, lesions, rupture of epithelium and degeneration of villi epithelium. The pathological changes were more severe at 7days exposure in both gill and intestine.