Molecular study to detection and genotyping of Entamoeba spp. In cattle & sheep.

The purpose of this study was planned to diagnose Entamoeba spp.in Cattles and Sheep By using the PCR method , for identified some of these species used the Nested -PCR, and identified genotypes of E. histolytica by RT-PCR and SYBER GreenI dye . Collected (48)Samples (24 from all cows and sheep ) that positive with microscopic test ,then examined with PCR to detection Entamoeba spp., species identified by Nested –PCR , then we determined genotypes of E. histolytica by amplification of SREHP gene used RT-PCR with SYBR GreenI dye and melting curve analysis, for the period from the beginning October 2014 until the end of March 2015 . This study recorded infection of cows and sheep by (E.histolytica; E.dispar ; E. bovis) with infection ratio as (78.5% , 28.5% , 21.5%) respectively and detected five genotypes (I , II ,III , IV , V) of E. histolytica, all genotypes infected cows but sheep infected with (II , V) only . Method of genotyping using SREHP gene and Melting curve analysis easy way and give guaranteed results, presence of five genotypes for SREHP in cows while two genotypes in sheep and the possibility of infect them with E.histolytica ; E.dispar and E.bovis which the first molecular recorder in Iraq.