Characterization of natural bee honey in different areas of the Sudan

Characterization investigation of five natural bee honey samples produced in different areas of Sudan, were carried out based on their physicochemical properties: pH, refractive index,moisture content, viscosity and free acidity ; in addition to sugars ,metals,ash and ascorbic acid contents. These included one sample from Darfur states, Kurdufan states, South Sudan, and another two samples from the Blue Nile State (amber and white honey). Sugar analysis was carried out with High Performance Liquid Chromatography using a refractive index detector (HPLC-RI). Metals analysis carried out by using Flame- Atomic Absorption Spectrometry (F-AAS). Ascorbic acid analysis was carried out using Ultraviolet-Visible spectrometry (UV-Vis). The results were showed significance difference between the different honey samples.