Callus cultivation and determination of some flavonoids by HPLC from some species of cucumber

The objectives of the current study were to evaluate the best medium for callus induction and regeneration of two species of Cucumis sativus L. (Waffir F1 and Fares). Evaluation of some flavonoids in tissue culture calli and tissue culture regenerated plantlets. Four explants (leaves, shoot tips, nodes and internodes) of cucumber cultivars (Waffir F1 and Fares ) cultured five weeks on MS medium supplemented with different combinations of two auxins (2,4-D and NAA) and two cytokinins (BA and Kin). Both of callus and regenerated plantlets of Waffir F1 cultivar was the most valuable and suitable for production of a great amounts of flavonoids through indirect regeneration systems.