Effect of Lactobacillus sp. Crude Bacteriocin (CB) and Cell-Free Supernatant (CFS) Against E.coli Growth and Adherence on Vaginal Epithelial Cell Surface.

Lactobacilli are gram positive bacteria, are naturally present as normal flora in the gastrointestinal tract, genital sewer and have several benefits, including: prevents infection of many pathogenic bacteria, e.g. Escherichia coli and Salmonella, helps fermentation of some foods such as different fermented milks, many industrial applications and have therapeutic properties against various infections and cancers. Cell-Free Supernatant (CFS) and Crude Bacteriocin (CB) have an anti-microbial effect that are produced by broad-spectrum of bacteria as lactobacillus; also have toxic properties to other strains of pathogenic bacteria, and other therapeutic properties. CFS and CB form a natural agents produced by probiotics live microorganisms, when administered in adequate amounts confers a health benefit on the host. So the objective of this study is to determine the effect of CFS and CB as natural agents produced from Lactobacillus sp. aginst E. coli growth and adherence on vaginal epithelial cell surface. The result showed loss ability to a growth of E. coli and form clear zone around culture after treated with serial concentration of CFS and CB, when a mixture of E. coli with human vaginal epithelial cells treated with a serial concentration of CFS and CB showed loss ability of bacteria to adherence on epithelial cell surfaces. So our study concluded that these products are very necessary to use as antibiotic alternative agents.