Characterization, Transformation and Stable Expression studies of Sucrose Synthase Gene promoter in Nicotiana Tabacum

The present study is focused on stable transformation using GUS expression cassette under selected sucrose synthase gene promoter in Nicotiana tabacum via Agrobacterium mediated transformation. The availability of a broad spectrum of promoters helps to regulate the temporal and spatial expression patterns of transgenes. The Agrobacterium mediated genetic transformation of plants is an important technique used to transfer foreign gene into the host plant genome. The expression of transgenes is regulated by the promoter attached upstream to the gene. Promoters are important in the control of the overall expression profile of a gene. The selected Sucrose Synthase gene promoter was previously cloned in plant expression vector (pGA482). Further, GUS expression cassette was verified through restriction analysis and PCR amplifications. The PCR positive tobacco transgenic plants for Sucrose Synthase expression cassette was obtained and stained to check the GUS expression levels.