Isolation and Characterization of Thermophilic Streptomyces Sp.with Potential Production of Actinokinase
Mohamed Abd Elmahamoud Ahmed
, Babiker Mohamed Babiker
, Aisha Mudawi Alawad
and Hanan Moawia Ibrahim
- Alnahda University. Faculty of Pharmacy, Sudan.
- National Ribat University. Faculty of Pharmacy. Sudan.
- Central Lab. Ministry of Higher Education & Scientific Research, Sudan.
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The study aimed to produce a potent fibrinolytic enzyme actinokinase from local isolate in batch fermentation, culture of identified thermophilic Streptomyces spp, was grown on glucose yeast extract peptone medium pH of 8.0. The hemolytic activity of the crude enzyme and the time spend for complete lysis was calculated using blood agar media, and test tubes containing clotted blood. The time of complete clot lysis in-vivo, using the crude actinokinase was found to be faster (20 min.). compared to the other commercial fibrinolytic enzyme (90 min.) The enzyme was stable at a broad pH ranging from 5 to 9. The thrombolytic potential of this particular isolate indicated that it could extract a promising actinokinase with potent activity.
[Mohamed Abd Elmahamoud Ahmed, Babiker Mohamed Babiker, Aisha Mudawi Alawad and Hanan Moawia Ibrahim (2016); Isolation and Characterization of Thermophilic Streptomyces Sp.with Potential Production of Actinokinase Int. J. of Adv. Res. 4 (Jan). 869-878] (ISSN 2320-5407). www.journalijar.com
