POSSIBLE ROLE OF HUMAN GLUTATHIONE PEROXIDASE-2 LEVEL IN PATIENTS SUFFERING FROM AMEBIASIS.

Background: Entamoeba histolytica, a microaerophilic protozoan parasite has to build up mechanisms to protect themselves against oxidative stress within the human gut to establish its pathogenesis, role of glutathione metabolism so important in amoebiasis but very little is known about mechanisms so this study aim to evaluate the level of human glutathione peroxidase-2 in the sera of patients suffering from amoebiasis. Methods: Blood samples were collected from 120 volunteer patients suffering from amoebiasis (62 females, 58 males) and compared with 50 healthy looking controls (25 females, 25 males). Attended to the central teaching hospital for pediatric in Baghdad city - Iraq, during the period between 1st March 2015 till 1st July 2015. The diagnosis was established based on direct microscopic examination of stool and the human glutathione peroxidase-2 concentaration has been evaluated based on quantitative sandwich enzyme immuno assay (Hcusabio- Cat. No. CSB-EL009867AU) . Results: We examined 120 children infected with Entamoeba histolytica, the infection in females 62 (55.66%) was higher than in males 58(48.33%). Highest prevalence of 57.50% (69/120) of Entamoeba histolytica infection was found in the age group of 10-16 years. The level of human glutathione peroxidase-2 was significantly lower in the patients than in control group. There were significant associations between the serum level of human glutathione peroxidase-2 and Entamoeba histolytica infection. Conclusion: Entamoeba histolytica infection had significant decreased on level of glutathione this related with utilizes by Entamoeba histolytica and used in detoxification processes.