The effect of L?carnitine on gentamicin-induced nephrotoxicity and associated anemia in adult male albino rats.

This study was conducted to investigate the possible ameliorating effect of L-carnitine against gentamicin-induced nephrotoxicity and some associated hematological changes in adult male albino rats and to exert the possible therapeutic role of L-carnitine depending on their antioxidant properities against the renal failure and its complication. A control group (n = 21) was compared with 80 mg/kg gentamicin treated rats, once daily for 10 days (n = 49). After 10 days 7 rats from each group were sacrificed for investigation. 42 nephrotoxic rats where further subdivided in to nephrotoxic rats (14 rats), nephrotoxic rats injected intraperitoneal with L-carnitine (300 mg/kg/day) for 15 and 30 days (14 rats), nephrotoxic rats injected intraperitoneal with L-carnitine (600 mg/kg/day) for 15 and 30 days (14 rats). At the end of each experiment period, 7 rats from each group were sacrificed. The effect of L-carnitine was detected by measuring urea, creatinine, β2-microglobulin, potassium (K), osmotic fragility, total oxidant status (TOS), iron (Fe), total iron binding capacity (TIBC) and ferritin. The effect of L-carnitine (group III and IV) was compared. The activities of biochemical parameters [urea, creatinine, β2-microglobulin, potassium (K), total oxidant status (TOS)] and [iron (Fe), total iron binding capacity (TIBC) and ferritin] increased in nephrotoxic rats, while total protein, sodium (Na) and total antioxidant status (TAS) decreased and in haematological parameters osmotic fragility increased but haemoglobin and red blood cells (RBCs) decreased in nephrotoxic rats. Administration of L-carnitine improved alterations of biochemical and haematological parameters. In conclusion, this study demonstrated that treatment with L-carnitine attenuated the biochemical and haematological alterations induced by gentamicin and identifies new areas of research for development of better therapeutic agents for kidney and better dose. increased in nephrotoxic rats, while total protein, sodium (Na) and total antioxidant status (TAS) decreased and in haematological parameters osmotic fragility increased but haemoglobin and red blood cells (RBCs) decreased in nephrotoxic rats. Administration of L-carnitine improved alterations of biochemical and haematological parameters. In conclusion, this study demonstrated that treatment with L-carnitine attenuated the biochemical and haematological alterations induced by gentamicin and identifies new areas of research for development of better therapeutic agents for kidney and better dose.