OPTIMIZATION CONDITIONS FOR THE PRODUCTION OF MICROBIAL TRANSGLUTAMINASE FROM A NEWLY ISOLATED STRAIN OF STREPTOMYCES SP.
- Food Science and Technology Department, Faculty of Agriculture, Alexandria University, El- Shatby, Alexandria, Egypt.
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The aim of the present study is to optimize the conditions for production microbial transglutaminase (MTGase) from Streptomyces diastaticus strain isolated from soil. The fermentation medium composition and the environmental conditions used for cell growth and enzyme production may be quite different. Therefore, it is important to optimize these parameters for the isolated strain. Three different fermentation media were tested to study the effect of the carbon sources on the enzyme production .It was found that the starch free medium is preferred than the other two media used (basal and glycerol containing media). The results indicated that the optimum conditions for the enzyme production were: the inoculum size of 8% of fermentation medium at temperature of 30ºC, pH 7.5 .The medium volumes was 100ml in fermentation flask of 600ml at 200 rpm.
[Eshra. D.H., Abo Bakr. T.M and El-Iraki, S.M. (2016); OPTIMIZATION CONDITIONS FOR THE PRODUCTION OF MICROBIAL TRANSGLUTAMINASE FROM A NEWLY ISOLATED STRAIN OF STREPTOMYCES SP. Int. J. of Adv. Res. 4 (May). 1722-1729] (ISSN 2320-5407). www.journalijar.com
