Phytochemical analysis and Biological studies of Indian medicinal plants Myristica fragrans and Tinospora cordifolia.

Phytochemical screening, isolation, antimicrobial and antioxidant activities of methanol, ethanol chloroform and water crude extracts of Myristica fragrans and Tinospora cordifolia were studied in this work. The aim of this work is to ascertain the level of antimicrobial activity and antioxidant properties of different solvent extracts of M. fragrans and T. cordifolia as well as isolated phytochemicals. The preliminary screening of various extracts was carried out using E. coli, C. sakazakii and P. aeruginosa by agar well diffusion method. The DPPH method used to determine the antioxidant potential of plant extracts and isolated phytochemicals. Myristicin and eugenol present in methanolic extracts exhibit direct relation with antimicrobial and antioxidant activity while palmatine and berberine could be responsible for T. cordifolia activities. The result showed that M. fragrans and T. cordifolia extracts have pharmocological active compounds with great antioxidant and antimicrobial effects. The results obtained from this study justify the use of this plant in traditional folk medicine and provide leads which could be further exploited for development of new and potent antimicrobials.