BIOSORPTION OF COPPER AND LEAD USING BACTERIAL BIOMASS OF BACILLUS CEREUS AND BACILLUS SUBTILIS ISOLATED FROM EL-MANZALA LAKE, EGYPT.
- National Institute of Oceanography and Fisheries, Inland Water and Aquaculture Branch, El-Qanater Research Station, Egypt.
- Botany and Microbiology Department, Al-Azhar University, Faculty of Science, Cairo, Egypt.
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Two tolerant bacterial strains isolated from El-Manzala Lake, Egypt and identified using biochemical tests and confirmed by 16S r RNA gene as Bacillus cereus and Bacillus subtilis.Then the optimum conditions for biosorption of copper and lead were investigated by using two bacterial strain, the equilibrium time for copper were 25 minutes at Bacillus cereusand 30 minutes at Bacillus subtilis while the equilibrium time for lead were 40 minutes at Bacillus cereusand 50 minutes at Bacillus subtilis, the optimum pH for copper and lead biosorption at Bacillus cereus and Bacillus subtilis was pH 6. The experimental biosorption data mostly were fitted towards the models postulated by Langmuir and Freundlich isotherm equations. The maximum biosorption capacities (q max) for copper and lead obtained by usingBacillus cereuswere 47.6 and 250 mg/g while by usingBacillus subtiliswere 166.7 and 250 mg/g , respectively. Biosorpitve mechanism was confirmed by IR analysis and from the identification nature of acidic and basic sites. Moreover, the postulated mechanism was depended mainly on ionic interaction and complex formation. The results demonstrated that the two bacterial isolates of Bacillus cereus and Bacillus subtilis could be used as a promising biosorbents for the removal of copper and lead ions from aqueous solutions.
[Shawky Z. Sabae ,Bahgt M. Refat and Usama M.Tahoun. (2016); BIOSORPTION OF COPPER AND LEAD USING BACTERIAL BIOMASS OF BACILLUS CEREUS AND BACILLUS SUBTILIS ISOLATED FROM EL-MANZALA LAKE, EGYPT. Int. J. of Adv. Res. 4 (May). 263-274] (ISSN 2320-5407). www.journalijar.com
