Detection of Hemolysins(hlyA and aerA) Genes in Aeromonas hydrophila Isolated from Raw and Drinking water in Baghdad City

A simplex and duplex PCR assay was used to amplify the Aeromonas hydrophila hemolysin and aerolysin genes. The assay was evaluated by using 63 A.hydrophila .A simplex PCR assay was performed to determine whether a597 bp hlyA gene and 416bp aerA gene fragments could be detected in sixty three Aeromonas isolates. Four genotypes among A. hydrophila isolates were found in this study ,the hlyA+ aerA+ genotype was the most common genotype.A survey was performed on the sixty three A. hydrophila isolates by duplex PCR to detect hemolysin genes presence. Four genotypes among A. hydrophila isolates also were found.When the presence of both toxin genes was investigated the hlyA+ aerA+ genotype was the most common.