Molecular and Immunopathological Role of Nuclear Factor K B Detected By Insitu Hybridization In Patho-genesis Of Chronic Atrophic Gastritis In Iraqi Patients

Chronic atrophic gastritis (CAG) characterized by chronic inflammation of gastric mucosa with loss of gastric glandular cells and replacement by intestinal-type epithelium and fibrous tissue. CAG may be associated with Helicobacter pylori infection. This study was designed to determine the role of NFkB signaling on Fas receptor mediated apoptosis in H.pylori Cag A+ associated CAG. According to exclusion criteria (45) CAG patients and (20) con-trol were chosen. Multiple gastric mucosal biopsies were taken for rapid urease test and preparation of slides from paraffin blocks. Slides stained with haematoxylin-eosin for histopathology and grading of PMNs and lymphocytes. Biotinylated DNA probe for human NF-KB and FAS genes were used for detection of their expression in tissue lympho-cytes, PMNs and gastric epithelial cells using ISH technique. Biotinylated DNA probe for H.pylori Cag A gene was used for detection of its expression in gastric epithelial cells. This study revealed that lymphocytes grade has positive correlation with Fas mRNA expression in PMN and gastric epithelial cells. PMN grade has positive correlation with tissue Fas mRNA; tissue NFkB mRNA; Cag A mRNA; lymphocytes and PMN NFkB mRNA expression. Lymphocytes Fas mRNA has positive correlation with PMN Fas mRNA and Cag A mRNA expression .Tissue Fas mRNA has positive corre-lation with tissue NFkB mRNA; Cag A mRNA ,lymphocytes and PMN NFkB mRNA expression. Tissue NFKB mRNA has positive correlation with lymphocytes and PMN NFkB mRNA expression. This study concludes that NFkB play vital role in Immunopathology of H.pylori Cag A+ associated gastritis that leads finally to atrophic changes.