SYNTHESIS, CHARACTERIZATION, ANTIMICROBIAL, CNS AND ANALGESIC STUDIES OF 2-[N-(4-chloro-2-{[1-(2-hydroxyphenyl) ethylidene]amino} phenyl) ethanimidoyl] phenol AND ITS COMPLEXES

4-Chloro-O-Phenylene diamine and its Copper (II) complex have been prepared by the Schiff base formation. The synthesized compound was confirmed from UV, IR & NMR spectrum. The O-Phenylene diamine and its Copper (II) complex found to have remarkable antimicrobial activity due to the presence of nitrogen group. The antimicrobial activities of three bactericidal organisms Bacillus subtilis, Streptococcus viridians and Staphylococcus epidermidis on the test compounds 4-Chloro-O-Phenylene diamine and its Copper (II) complex were screened by employing the Disc Diffusion method. A concentration gradient (500, 1000, 2000 and 3000?g) of each compound was put into study. From the study, it is observed that its shows a maximum zone of inbition. Chapter I is the introduction which deals with the introduction of Schiff base and its complex, Ultraviolet and visible spectroscopy, infrared, NMR spectroscopy of the complexes, biological importance of Schiff base complexes and its applications. Chapter II describes the literature review about O-phenylene diamine and 2-hydroxy acetophenone, nature of ligand, structure of ligand, nuclearity and its geomentry Chapter III describes the scope of the present work, synthesis, antimicrobial study, CNS and analgesic study Chapter IV describes the scheme of the present work which deals with the synthesis of the ligand and its complex Chapter V describes the experimental section which consists of chemicals required, materials required and synthesis of ligand and its complex comprising derivatives of 4-Chloro-O-Phenylene diamine and 2-hydroxy acetophenone, experimental procedure for antibacterial activity, CNS study and analgesic study Chapter VI describes the confirmation of synthesized ligand and its complex by TLC, UV, FT-IR, NMR spectroscopy, antimicrobial studies by disc diffusion method, CNS studies using digital actophotometer, analgesic studies using analgesiometer and its graph. The antibacterial studies against Gram positive Bacillus subtilis, Streptococcus viridians and Staphylococcus epidermidis were determined by disc diffusion method and maximum zone of inhibition was calculated