MULTIPLICATION OF NEMAGUARD AND MARIANA ROOTSTOCKS: INFLUENCE OF CYTOKININS AND ADENINE SULFATE AND THEIR CONCENTRATIONS.

This study was conducted during the period 2015 to 2016 at plant tissue culture laboratory, Department of Horticulture, College of Agriculture, University of Baghdad. In this research, the effect of cytokinin (6-Benzyladenin (BA) and Kinetin (Kin)), Adenine Sulfate (AS) and their concentrations on shoot multiplication were evaluated. Young nodal segments with a single bud from 5-8 years old of peach and plum rootstocks (Nemaguard and Mariana) were used as explants. The results can be summarized as follows: For Nemaguard and Mariana shoot induction, MS (Murashige and Skoog, 1962) medium supplemented with (BA) 2mgl-1 and 0.2mgl-1 GA3 (Gibbrelic Acid) and 0.4mgl-1 (GA3), resulted in high shoot regeneration percentage (100%). For shoot multiplication, 6mgl-1 (Kin) in combination with 50 mgl-1 (AS) increased the mean of shoots number up to 13.62, 15.62 shoot per explant for Nemaguard and Mariana respectively. From the tested combination 6mgl-1 (Kin) with 50 mgl-1 (AS) was best performing by resulting in 4.20 cm mean length of shoots for Nemaguard rootstock.