Using Polymerase Chain Reaction ( PCR) Technique For Detection E .coli O157:H7 BacteriaFrom Raw Cow's Milk and Local SOFT Chesses.

E.coliO157:H7 has emerged as an important gastrointestinal pathogen of man can cause severe illness and death. The present study was undertaken to detect the E.coliO157:H7 in 125 samples (75 raw milk,25 pasteurized milk and 25 soft Arab chesses ). Samples were collected from deferent location (source) in Baghdad city (Abu Ghraib, Al Kadhimiya, Al-A'amiriya and Fudaliyah). During the period from October 2015toJanuary 2016. All samples were screened to detect the presence of non-sorbitol fermenting colonies (NSF) on sorbitol MacConkey agar supplemented with Cefixime, Pastorium tolerate (CT-SMAC). A total of 125 samples, 28 (37.3%) from raw milk, 0 (0%) from pasteurized milk and 14 (56%) from soft Arab cheese (NSF), with chromogenic media 10(13.3%) from raw milk, and 4 (16%) from soft Arab chesses which appear purple on the media, E. coli isolates were serotyped as E. coli O157:H7 by latex agglutination test, 7 (9.3%) isolates of raw milk, and 2 (8%) isolates of soft Arab chesses. All the latex agglutination positive isolates were positive to result of the vitek2 test. The specificity of the primers was evaluated byspecific primers (uidR and flich7 genes) 7(9.3%) 2(8 %) isolates of rawmilk, and soft Arab chesses samples respectively. In this study was evaluate based on Polymerase chain reaction (PCR) technique, that amplified the uidR and flicH7 genes.