A QUICK AND EASY METHOD FOR THE ISOLATION OF PERIPHERAL BLOOD MONONUCLEAR CELLS FROM WHOLE BLOOD BY DENSITY GRADIENT CENTRIFUGATION USING HISEP LSM MEDIUM.

Isolation of peripheral blood mononuclear cells (PBMCs) from whole blood is most commonly accomplished through density gradient centrifugation using density gradient solution such as ficoll paque. In this study, we describe a simple and rapid method for PBMC isolation using Hisep LSM as density gradient. High numbers of fully functional cells are obtained from whole blood and analyzed by flow cytometer and inverted microscope. The results of isolated PBMCs are reported in which the yield of cells are 3.6 × 106 cells/ml with 98.92% viability by tryphan blue exclusion method and are 1.46×106 cells/ml with 95.35% viability by flow cytometric analysis. This technique is a convenient, quick, relatively simple, and inexpensive alternative to traditional PBMCs isolation techniques.