Vol. 5 (05) pp. 950-955 DOI: 10.21474/IJAR01/4203

CORRELATION BETWEEN THE GENOTYPE DETECTION DETERMINED BY RT- PCR ASSAYS AND THE PHENOTYPIC DETECTION DETERMINED BY ANTIBIOTIC SUSCEPTIBILITY PATTERNS OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS CLINICALISOLATES IN ALGERIA.

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  • Laboratoire de Biologie Cellulaire et Mol?culaire, Facult? des Sciences de la nature et de la vie, Universit? des fr?res Mentouri Constantine1, Rue Ain El bey- 25000 Constantine-Alg?rie.
  • Laboratoire de Microbiologie, H?pital de p?diatrie d'El-Mansourah, Constantine-Alg?rie.
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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a major public health problem causing both hospital and community associated infections worldwide. Methicillin resistance is usually due to the mecA gene that codes for a 78-kD a penicillin binding protein (PBP2a), with decreased affinity to methicillin and all beta-lactam antibiotics. A total of 65 S. aureus isolates were collected from a pediatric hospital and clinical laboratories in Constantine, from December 2014 to September 2015. All isolates were identified using matrix-assisted laser desorption/ionisation time of flight mass spectrometry(MALDI-TOF-MS). The phenotypic detection of methicillin-resistant was investigated using the standard disk-diffusion method on Mueller?Hinton agar. The isolates were tested with 16 antibiotics: Penicillin, oxacillin, Cefoxitin, Ceftriaxone, Ciprofloxacin, Gentamicin, Teicoplanin, Vancomycin, Erythromycin, Clindamycin, Pristinamycin, Doxycycline, Fosfomycin, Linezolid, Rifampicin and Trimethoprim-sulfamethoxazole. They were incubated at 37?C for 24 h. Results showed that 35.38% of isolates were methicillin-resistantStaphylococcus aureus. According to the obtained results from RT-PCR analysis of methicillin-resistant S. aureus (MRSA), mecA gene was present in 86.95% of the resistant isolates. The results of comparison between RT-PCR detection of mecA gene and standard disk diffusion test showed high degree of correlation.

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How to Cite This Article

Amina Ayeche, N?vine Lebsir And Mohamed Abdelhafid Hamidechi. (2017); CORRELATION BETWEEN THE GENOTYPE DETECTION DETERMINED BY RT- PCR ASSAYS AND THE PHENOTYPIC DETECTION DETERMINED BY ANTIBIOTIC SUSCEPTIBILITY PATTERNS OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS CLINICALISOLATES IN ALGERIA., Int. J. of Adv. Res., 5 (05), 950-955, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/4203

Corresponding Author

Amina AYECHE
Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences de la nature et de la vie, Université des frères Mentouri Constantine1, Rue Ain El bey- 25000 Constantine-Algérie.
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.