Vol. 5 (10) pp. 1052-1060 DOI: 10.21474/IJAR01/5625

OPTIMIZATION OF LIPASE ACTIVITY FROM STAPHYLOCOCCUS GALLINARUM AND ITS PARTIAL PURIFICATION.

and
  • Department of Microbiology, Guru Nanak Khalsa College, Matunga, Mumbai-19, Maharashtra, India.
32 Downloads 130 Views
Crossref

Download Full Paper (PDF)

Abstract

Lipolytic enzymes from microbial sources have been explored in budding research in various arenas. A lipase producing bacterium was isolated from spoilt butter and identified as Staphylococcus gallinarum. The present study was carried out to maximize activity of enzyme lipase from Staphylococcus gallinarum. For this, variations in carbon, nitrogen and oil sources of original glucose yeast extract broth supplemented with oil were carried out. High lipase activity was observed with 2% glucose, 1% peptone, 1 % olive oil, 0.15 optical density at pH 7.0, 37 ?C at 200rpm. Partially purified enzyme with 8329.6 U/mg specific activity, 29.6 fold purification and 19.22 % yield was obtained by fractional ammonium sulphate precipitation method.

Keywords

Article Analytics

References

  1. Abbas, H., & Comeau, L. (2003). Aroma synthesis by immobilized lipase from Mucor sp. Enzyme and Microbial Technology, 32(5), 589?595.
  2. Adinarayana, K., Bapi Raju, K. V. V. S. N., Iqbal Zargar, M., Bhavani Devi, R., Jhansi Lakshmi, P., & Ellaiah, P. (2004). Optimization of process parameters for production of lipase in solid-state fermentation by newly isolated Aspergillus species. Indian Journal of Biotechnology, 3(January), 65?69.
  3. Ananthi, S., Ramasubburayan, R., Palavesam, A., & Immanuel, G. (2014). Innovare Academic Sciences Optimization and purification of lipase through solid state fermentation by bacillus cereus msu as isolated from the gut of a marine fish sardinella longiceps. International Journal of Pharmacy and Pharmaceutical Sciences, 6(5).
  4. Benjamin, S., & Pandey, A. (2001). Isolation and characterization of three distinct forms of lipases from candida rugosa produced in solid state fermentation. Brazilian Archives of Biology and Technology, 44, 213?221.
  5. Brault, G., Shareck, F., Hurtubise, Y., L?pine, F., & Doucet, N. (2014). Short-chain flavor ester synthesis in organic media by an E. coli whole-cell biocatalyst expressing a newly characterized heterologous lipase. PloS One, 9(3), e91872.
  6. Croitoru, R., Broek, L. A. M. Van Den, Frissen, A. E., Davidescu, C. M., Peter, F., & Boeriu, C. G. (2011). Lipase Catalyzed Synthesis of Aromatic Esters of Sugar Alcohols. International Journal of Chemical, Molecular, Nuclear, Materials and Metallurgical Engineering, 5(4), 332?337.
  7. Denise, S., Livia, T. A. S., Jamil, S. O., Marcelo, M. S., Inayara, C. A. L., Gecernir, C., & Jacqueline, A. T. (2015). Colletotrichum gloeosporioides lipase: Characterization and use in hydrolysis and esterifications. African Journal of Microbiology Research, 9(19), 1322?1330.
  8. Imandi, S. B., Karanam, S. K., & Garapati, H. R. (2013). Use of Plackett-Burman design for rapid screening of nitrogen and carbon sources for the production of lipase in solid state fermentation by Yarrowia lipolytica from mustard oil cake (Brassica napus). Brazilian Journal of Microbiology, 44(3), 915?921.
  9. Kocharekar, A. M., & Dukhande, M. S. (2017). Paper Microfluidics in Microbial World. International Journal of Science and Research (IJSR), 6(3), 1909?1914.
  10. Longo, M. A., & Sanrom?n, M. A. (2006). Production of food aroma compounds: Microbial and enzymatic methodologies. Food Technology and Biotechnology, 44(3), 335?353.
  11. Lux, L., Valeriano, L., Corbellini, A., & Valente, P. (2007). Lipases produced by yeasts : powerful biocatalysts for industrial purposes. TECNO-LOGICA, 18?25.
  12. Ortansa csutak, ileana stoica, T. V. (2015). Lipolytic activity of a new yeast strain Candida rugosa CMGB-CR6 isolated from oil-polluted soil. Romanian Biotechnological Letters, 20(3), 10547?10557.
  13. Padmapriya, B., Rajeswari, T., Noushida, E., Sethupalan, D. G., & Venil, C. K. (2011). Production of lipase enzyme from Lactobacillus spp. and its application in the degradation of meat. World Applied Sciences Journal, 12(10), 1798?1802.
  14. Ramesh, S., Kumar, R., Devi, R. A., & Balakrishnan, K. (2014). Original Research Article Isolation of a lipase producing bacteria for enzyme synthesis in shake flask cultivation. J.Curr.Microbiol.App.Sci, 3(3), 712?719.
  15. Rekha, K. S. S., Lakshmi, M. V. V. C., Devi, V. S., & Kumar, M. (2012). Production and Optimization of Lipase From Candida Rugosa Using Groundnut Oilcake Under Solid State, 571?577.
  16. Similar, C. (2004). Chapter 3 results and discussion Part 1 . Synthesis of Sugar Esters from Palm Oil and Palm Fatty Acid Distillates using Commercial Lipases, 61?148.
  17. Veerapagu, M., Sankara Narayanan, , Ponmurugan, K., & Jeya, K. R. (2013). Screening selection identification production and optimization of Bacterial Lipase from oil spilled soil. Asian Journal of Pharmaceutical and Clinical Research, 6(SUPPL.3), 62?67.
  18. Yapasan, E., & Yalcin, A. P. D. R. T. (2008). Partial purification and characterization of lipase enzyme from a Pseudomonas strain. School of Engineering and Science, Master of(December), 49.

How to Cite This Article

Amita Madhukar Kocharekar and Meenal Shriniwas Dukhande. (2017); OPTIMIZATION OF LIPASE ACTIVITY FROM STAPHYLOCOCCUS GALLINARUM AND ITS PARTIAL PURIFICATION., Int. J. of Adv. Res., 5 (10), 1052-1060, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/5625

Corresponding Author

Ms. Amita Madhukar Kocharekar
PhD scholar, Department of Microbiology, Guru Nanak Khalsa College, Mumbai, India.
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.