Vol. 5 (11) pp. 818-823 DOI: 10.21474/IJAR01/5835

MOLECLAR CHARACTERIZATION OF SURFACE ADHESINS OF UROPATHOGENIC ESCHERICHIA COLI FROM PATIENTS IN A TERTIARY CARE HOSPITAL.

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  • Department of Microbiology, Shri Sathya Sai medical college, Tamil Nadu, India.
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Abstract

Uropathogenic E.coli (UPEC) classified under Extraintestinal pathogenic E.coli (Expec) with multiple virulence markers encodes for surface adhesive organelles. The study was carried out to isolate and to identify E.coli from urine samples and to characterize the adhesive genes by molecular technique - pap (pyelonephritis associated pili), fimH (type 1 fimbriae) and sfa (S fimbrial adhesin).A total of 96% out of 208 E.coli were positive for pap, fimH and sfa genes with varied frequencies - pap 46% (96/208), fimH 92% (192/208) and 58% (28/208) sfa genes. 12.5% of isolates possess all three adhesive genes. The antibiotic pattern showed resistance to nalidixic acid (80.8%), Ceftazidime (49.5%), Cefepime (47.6%), Gentamicin (20.2%), Norfloxacin (30.8%). Fimbrial adhesion may lead to resistance towards host defence, biofilm formation resulting in relapse and recurrences of infection. Molecular studies help us to determine the multiple virulence genes and to understand the complexity and severity of UTI.

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How to Cite This Article

Nachammai SM and Dr Karthicka Jayakumar. (2017); MOLECLAR CHARACTERIZATION OF SURFACE ADHESINS OF UROPATHOGENIC ESCHERICHIA COLI FROM PATIENTS IN A TERTIARY CARE HOSPITAL., Int. J. of Adv. Res., 5 (11), 818-823, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/5835

Corresponding Author

Nachammai SM
Shri Sathya Sai Medical College
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.