Vol. 7 (09) pp. 1169-1177 DOI: 10.21474/IJAR01/9769

EFFECTS OF PHYSICAL PARAMETERS AND ALGORITHM OF PETROLEUM HYDROCARBONS UTILIZATION BY SOME BACTERIAL ISOLATES FROM BONNY, RIVERS STATE NIGERIA.

, , , , and
  • Department of Biotechnology, Federal University of Technology, Owerri, P.M.B. 1526 Owerri, Imo State, Nigeria.
  • Department of Biology, Federal University of Technology, Owerri, P.M.B. 1526 Owerri, Imo State, Nigeria.
14 Downloads 121 Views
Crossref

Download Full Paper (PDF)

Abstract

The ability of some bacterial isolates to utilize crude oil and the effects of pH and various nitrogen sources on the growth and utilization of hydrocarbons were investigated. A total of six bacterial isolates were isolated. These include two different species of Bacilli, three different species of Pseudomonas and Klebsiella species. The effects of pH on the growth of the isolates showed that Pseudomonas sp (A2) had the highest growth of about 2.25?105 cfu/ml at pH 6.5. Baciliussp (A5), Klebsiellasp (A1) recorded growths of 2.04?105 and 1.70?105 cfu/ml at pH 7.5 and 7.0 respectively. These isolates were able to utilize and grow on different nitrogen sources containing ammonium sulphate((NH4)2SO4), sodium nitrate (NaNO3), and ammonium chloride (NH4Cl). NH4Cl was the best utilized nitrogen source with growth of 2.37?105, 2.17?105, and 1.93?105 cfu/ml for Pseudomonas, Bacillus and Klebsiella species respectively. The effects of pH on the utilization of Bonny light crude oil revealed percentage utilization of about 76%, 68%, and 58% at pH of 6.5, 7.5 and 7.0 respectively for Pseudomonas, Bacillus and Klebsiella species. Percentage utilization of about 67%, 63%, and 51.8% were recorded for Pseudomonas, Bacillus and Klebsiella species when grown on mineral salt media supplemented with NH4Cl. The isolates were tested for their ability to utilize bonny light crude oil using the gravimetric method. The percentage utilization of all the isolates ranged between 77.5%, 66.8%, and 54.2% after 28 days of incubation for Pseudomonas, Bacillus and Klebsiella species respectively.

Keywords

How to Cite This Article

Ezeji E. U., AnyanwuC. O., Onyeocha I. O., Chukwudi P., Ukaoma A. A. and UkwanduN. C. D. (2019); EFFECTS OF PHYSICAL PARAMETERS AND ALGORITHM OF PETROLEUM HYDROCARBONS UTILIZATION BY SOME BACTERIAL ISOLATES FROM BONNY, RIVERS STATE NIGERIA., International Journal of Advanced Research (IJAR), 7 (09), 1169-1177, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/9769

Corresponding Author

Ezeji EU
Federal University of technology Owerri, Nigeria

Article Analytics

References

  1. Adeline, S. Y., Ting, C., Tan, H. C. & Aw, C. S. (2009). Hydrocarbon-degradation by isolate Pseudomonas lundensis UTAR FPE2. Malaysian Journal of Microbiology 5 (2): 104-108.
  2. Ainon, H., Amir, R., Raja, f., Hanim, , & Noor,? A. (2010). Isolation and Characterization of Bacteria Degrading Sumandak? and South Angsi Oils. SainsMalaaysian, 39(2), 161?168.
  3. Antai, S., &Mgbomo, E. (1989). Distribution of hydrocarbon utilizing bacteria in oil spill areas. Microbiological Letter, 8: 137-143.
  4. Atlas, R. (1981). Microbial degradation of petroleum hydrocarbons; an experimental perspective. Microbiological Reviews, 45(1), 180-209.
  5. Banat, I. M., Makkar, R., &Cameotra, S. (2000).?Potential commercial applications of microbial surfactants. Applied Microbiology and Biotechnology, 53: 495-508.
  6. Bossert I., & Bartha, R. (1984). The fate of petroleum in soil ecosystem. In: Petroleum Microbiology. Macmillan, New York. 435-475.
  7. Brooijmans, R., Pastink, M. and Siezen, R. (2009). Hydrocarbon-degrading bacteria: the oil-spill clean-up crew.Microbial Biotechnology, 2: 587?594.
  8. Das, K., & Mukherjee, A. (2007). Comparison of lipopeptidebiosurfactants production by Bacillus subtilis strains in submerged and solid state fermentation systems using a cheap carbon source: some industrial applications of biosurfactants.Process?Biochemistry, 42: 1191?1199.
  9. Ebuehi, O. A. T., Abibo, I B., Shekwolo, P D.,?Sigismund, K. I.,?Adoki, A &Okoro, I C. (2005). Remediation of Crude Oil Contaminated Soil By Enhanced Natural Attenuation Technique. Journal of Applied Sciences & Environmental Management, 9(1), 103-106.
  10. Eniola, K., Adegbola, G., &Opasola, O. (2014). Biodegradation of Used Engine Oil By Bacteria Isolated From Soil Contaminated With Used Engine Oil in Ogbomoso, Nigeria. Journal of Environmental Science, Toxicology and Food Technology, 8(5), 66-70.
  11. Essien, J., Ubom, R., &Udosen, E. (1997). Bioremidation of petroleum contaminated soil; Effect on the population dynamics and degrading capabilities of hydrocarbonoclastic bacteria. Journal of applied biological chemistry, 43: 22-28.
  12. Ezeji, E. U., Anyanwu, B. N., Onyeze, O. G., &Ibekwe, I, V. (2005). Studies on the utilization of Petroleum Hydrocarbon by Micro Organism isolated from oil Contaminated Soil. International Journal?of?Applied?and Natural Sciences, 1(2), 122-128.
  13. Gerson, D. (1985). The Biophysics of Microbial growth on hydrocarbon: Insoluble substrates. International Bioresource?Journal, 1: 39-53.
  14. Hildebrandt, W., & Wilson, S. (1991). On-site bioremediation systems reduce crude oil contamination. Journal of Petroleum Technology, 43(1), 18-22.
  15. Kalaivani, N., & Tan, S. T. (2013). Isolation and identification of catechol degrading bacteria. Journal of Pure and Applied Microbiology, 7(2), 881-890.
  16. Nwaogu, L., Onyeze, G., &Nwabueze, R. (2008). Degradation of diesel oil in a polluted soil using Bacillus subtilis. .African Journal of Biotechnology, 7(12), 939-1943.
  17. Ohta, Y., Maeda, M., & Kudo, T. (2001). Pseudomonas putida CE2010 can degrade biphenyl by a mosaic pathway encoded by the tod operon and cmtE, which are identical to those of P. putida F1 except for a single base difference in the operator?promoter region of the cmt operon. Microbiology, 147: 31-41.
  18. Onugbolu, C. &Adieze I. (2016). Assessment of emulsification potentials of hydrocarbon utilizing fungi isolates isolated from a crude oil polluted at FUTO, Owerri. Nigerian Journal of Microbiology, 30(1), 3225-3234.
  19. Salam, L. B., Obayori, O. S., Akashoro, O. S., &Okogie, G. O. (2011). Biodegradation of bonny light crude oil by bacteria isolated from contaminated soil. International Journal of Agriculture and Biology, 13: 245?250.
  20. Sidkey, N. M., Mohamed, H., &Elkhouly. (2016). Evaluation of different screening methods of biosurfactant producers isolated from contaminated egyptaian samples grown on industrial olive oil processing waste. British Microbiology Research Journal, 7(4), 1-19.
  21. Mu?oz, R., Rojas, , D?az,? L., Bordel, S., &Villaverde, S. (2007). Inhibitory effects of catechol accumulation on benzene biodegradation in Pseudomonas putida F1 cultures. Chemosphere, 68: 244-52.
  22. Udotong, I. (1995). Petroleum exploration and production (E&P) ?derived waste stream management in the Nigerian oil industry.Journal of Scientific Engineering and Technology,2(2), 201-217.
  23. Yu, H., Kim, B., &Rittmann, B. (2001). The roles of intermediates in biodegradation of benzene, toluene, and p-xylene by Pseudomonas putidaF1. Biodegradation, 12: 455-63.
  24. Yuan, S., Shiung, L., & Chang, B. (2002). Biodegradation of polycylic aromatic hydrocarbons by inoculated microorganisms in soil. Bulletin of Environmental Contamination and Toxicology, 69: 66-73.

Similar Articles

Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.