FTIR-ATR SPECTRAL AND BIOCHEMICAL VARIATIONS IN DIABETES MELLITUS INDUCED BY ALLOXAN IN WISTAR MALE RAT.

Diabetes Mellitus (DM) is a destructive disease of carbohydrate, fat and protein metabolism affecting a great amount of world’s population. The experiment have been made on the White Wistar rats and recipient rats are injected with alloxan ( 40 mg/kg body weight ) subcutaneously. The experiment have developed DM with Clinical symptoms of haematuria, hyperglycemia etc.,. Blood glucose values were 489 ± 8.48 mg/dl recorded to assess the DM in experimental animals induced with alloxan. Current study focus on FTIR spectroscopy together with routine techniques as a diagnostic tool in the discrimination of diabetic samples from healthy ones . A variety of alterations in the spectral parameters, such as frequency and signal intensity/area was observed in diabetic rat compared to the control samples. Based on these spectral variations and routine analysis successful differentiation between diabetic and control groups was obtained in different spectral regions. The results of this current study further revealed the power and sensitivity of FTIR spectroscopy in precise and automated early diagnosis of diabetes. FTIR-ATR spectral variations and quantification of bio molecules among control and experimental animals observed are statistically more significant . The importance of FTIR –ATR Spectroscopy technique in evaluation of qualitative and quantitative nature of biochemical components is discussed.