IN-VITRO ANTIDIABETIC ACTIVITY OF SWIETENIA MAHAGONI (L). SEEDS AND ITS DIFFERENT FRACTION WITH ISOLATED COMPOUNDS

Active compounds of Swietenia mahagoni seed have power as anti diabetic that can be used in diabetes mellitus treatment. S. mahagoni seeds were extracted using methanol-maceration, followed by partitioning with petroleum ether (PE), chloroform (CL), ethyl acetate (EA), and dia-ion resin (DR). Column chromatography was used to further separate the dia-ion Resin fraction in order to get isolate purer compounds. One of the strategies is maintaining postprandial glucose level through inhibition of α-amylase and α-glucosidase. So that pre-prandial and post-prandial glucose levels can be controlled properly. The aim of this study was to determine in vitro inhibitory activity of Swietenia mahagoni seed by α-glucosidase, α-amylase. Inhibitory activity was measured using spectrophotometric method, α-amylase activity was measured at λ = 540 nm, α-glucosidase activity measured at λ = 405 nm. DRF inhibit both α-glucosidase and α-amylase better than acarbose as a positive control. This study showed that some extracts and some isolated compounds have inhibition activity of α-glucosidase and α-amylase. According to the findings, DR fraction (IC-50 = 328.22μg/mL) had a higher level of alpha-glucosidase inhibitory activity than acarbose (IC-50 = 336.95μg/mL). The six DR fractions compounds were separated using column chromatography. The DR fraction (40.62%), (50.39%), showed the highest inhibition activity.